Title Human IgG1 expression in silkworm larval hemolymph using BmNPV bacmids and its N-linked glycan structure
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منابع مشابه
Human IgG1 expression in silkworm larval hemolymph using BmNPV bacmids and its N-linked glycan structure.
A Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid expressing heavy and light chains of human 29IJ6 IgG was constructed and used to secrete recombinant antibody into silkworm larval hemolymph. Fifth instar silkworm larvae were reared and injected into the dorsum of the larvae with recombinant cysteine protease- and chitinase-deficient BmNPV (BmNPV-CP(-)-Chi(-)) bacmid/29IJ6 IgG and harvested aft...
متن کاملHuman single-chain antibody expression in the hemolymph and fat body of silkworm larvae and pupae using BmNPV bacmids.
Seven sets of recombinant expression vectors were constructed for the expression of the human anti-bovine serum albumin (BSA) single-chain Fv fragment (scFv) 13CG2 fused to the C terminus of GFP(uv) or bombyxin (bx) signal peptide in the hemolymph and fat body of silkworm larvae and pupae, using cysteine protease- (BmNPV-CP(-)), and cysteine protease- and chitinase-deficient (BmNPV-CP(-)Chi(-))...
متن کاملIgG in Silkworm, and No Alterations in their N-Linked
Human 29IJ6 IgG was expressed in silkworm using a Bombyx mori 25 nucleopolyhedrovirus (BmNPV) bacmid system. The mean amounts of 296IJ6 IgG 26 produced in larval hemolymph and whole pupae were 30.1 μg/larva and 78.0 μg/pupa,
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BACKGROUND Baculovirus, which has a width of 40 nm and a length of 250-300 nm, can display functional peptides, receptors and antigens on its surface by their fusion with a baculovirus envelop protein, GP64. In addition, some transmembrane proteins can be displayed without GP64 fusion, using the native transmembrane domains of the baculovirus. We used this functionality to display human proreni...
متن کاملHigh-titer preparation of Bombyx mori nucleopolyhedrovirus (BmNPV) displaying recombinant protein in silkworm larvae by size exclusion chromatography and its characterization
BACKGROUND Budded baculoviruses are utilized for vaccine, the production of antibody and functional analysis of transmembrane proteins. In this study, we tried to produce and purify the recombinant Bombyx mori nucleopolyhedrovirus (rBmNPV-hPRR) that displayed human (pro)renin receptor (hPRR) connected with FLAG peptide sequence on its own surface. These particles were used for further binding a...
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